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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been noticed in many tumors, which ends up in chromosome instability, and so may contribute to tumorigenesis. This protein was discovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless available.

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Blocking peptides are peptides that bind specifically for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded with the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This information outlines additional feasible optimization of cellular and immunoassay parameters to be certain the best possible final results are...

Antibodies sure to the blocking peptide now not bind into the epitope around the target protein. By comparing the staining through the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.

Target Information and facts Cyclin E belongs on the highly conserved cyclin relatives, whose associates are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation designs which add to the temporal coordination of every mitotic occasion. Cyclin E varieties a complex with and features to be a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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